Presence of Human Papillomavirus in Dentigerous Cyst and Ameloblastoma (HPV in Dentigerous Cyst and Ameloblastoma)

Aims: The aim of the current study was to detect and compare Human papillomavirus (HPV) in dentigerous cyst and ameloblastoma. Materials and methods: The study included 32 samples, which distributed into dentigerous cysts (n=16) and ameloblastomas (n=16). Conventional PCR assay using SPF1/2 consensus primers and immunohistochemical analysis were performed for the detection of HPV‒DNA and HPV‒L1 protein respectively. Results: HPV‒DNA was detected in 25% (4/16) of dentigerous cyst samples and in 45.45% (5/11) of ameloblastomas samples, whilst HPV‒L1 protein expression was identified in 25% (4/16) of dentigerous cysts and in 75% (12/16) of ameloblastomas. Chi‒square test showed significantly higher expression of HPV‒L1 protein in ameloblastomas than in dentigerous cysts (P<0.01). Kappa coefficient showed moderate agreements (k=0.526) between PCR and immunohistochemical results. Conclusions: The current study concludes that both HPV‒DNA and HPV‒L1 protein could be detected in ameloblastomas and to a lesser extent in dentigerous cysts. This may indicate a possible relation of HPV with those lesions.